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Some experiments require only a qualitative answer, but properly designed and executed western blot experiments can also provide quantitative data on relative protein expression between samples. Find a comprehensive suite of solutions for every step of the western blotting workflow in order to help you obtain high-quality, publishable results with minimal time and effort. Find the right set of products that can assist you with detection and analysis of your protein. Understanding basic imaging concepts such as sensitivity, resolution, and sources of background noise can help you maximize image data quality.Īn image of a western blot is rich with information. Western blotting is an invaluable tool for protein biology. This mixture can include all of the proteins associated with a particular tissue. In addition to detection of specific proteins, the total protein in a sample can also be visualized with total protein stains or newer technologies that eliminate the need for staining and destaining.Īccurate imaging of your western blot is crucial for capturing blot data for downstream analysis. A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. Efficient protein transfer is required for maximum western blot sensitivity.Ī good, clean western relies on the specificity and sensitivity of your antibodies. Separated proteins are transferred from the gel to a membrane where they are immobilized. Powerful algorithms are designed to consistently and accurately select and identify lanes and band boundaries for molecular weight estimation, densitometry, or normalization. Good sample preparation techniques ensure proteins remain undamaged for downstream analysis.Įlectrophoresis separates the proteins in the sample and provides molecular weight data for detected proteins during subsequent detection. Automatically analyze up to four mini blots or mini gels at a time from a single image for increased throughput. Cells containing your protein of interest must be lysed completely to ensure a high yield while removing non-protein components of cells.